Coding
Part:BBa_K566007:Design
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-26)
PenI repressor optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites was performed, they were found and eliminated.
Source
Original sequence was taken from Registry (BBa_C0074), this was optimized with preferential codons for better expression in E. coli.
References
1. Wittman V, Lin HC, Wong HC (1993) Functional domains of the penicillinase repressor of Bacillus licheniformis. J Bacteriol 175:7383-7390